Can the medium for M2 macrophages be switched to RPMI or RPMI + M‑CSF after differentiation?
This has not been tested by PromoCell and would need to be evaluated by the customer.
PromoCell’s M2 Macrophage Generation Medium XF is substantially different from RPMI‑based media. Therefore, we cannot predict whether switching to RPMI or RPMI + M‑CSF after differentiation would maintain cell viability, phenotype, or function.
Based on our experience, successful long‑term culture of M2 macrophages has only been validated within the PromoCell system using our media.
PromoCell’s M2 Macrophage Generation Medium XF is substantially different from RPMI‑based media. Therefore, we cannot predict whether switching to RPMI or RPMI + M‑CSF after differentiation would maintain cell viability, phenotype, or function.
Based on our experience, successful long‑term culture of M2 macrophages has only been validated within the PromoCell system using our media.
Products relevant to this topic
M2-Macrophage Generation Medium XF
Cat No
C-28056
Serum-free and xeno-free medium for generation of M2 macrophages from fresh monocytes.
1,109.00$
Human M2 Macrophages (M-CSF), Monocyte-derived, single donor, 1,5 Mio cells
Cat No
C-12915
Monocyte-derived, polarized, non-activated, primary human macrophages. Polarization in M2-Macrophage Generation Medium XF (incl. M-CSF).
1,332.00$