Air-Liquid Interface Medium

Our Air-Liquid Interface Medium (ALI-Airway) is a serum- and bovine pituitary extract (BPE)-free cell culture medium for the standardized generation of a 3D airway model with a reproducible epithelial barrier function using Human Bronchial Epithelial Cells (HBEpC). The medium contains a low level of growth factors to maintain a non-proliferating cell population and it suits perfect for long-term culture on a semipermeable membrane. The functionality of the airway epithelial cells can be monitored by a fast development of an epithelial barrier formed by tight junctions.

In addition to the ALI-Airway medium, we provide HBEpCs pre-screened for epithelial barrier formation in our ALI-Airway medium. When culturing ALI pre-screened HBEpC in our ALI-Airway Medium, we guarantee a stable transepithelial electrical resistance (TEER) of > 500 Ω*cm² over a time period of at least 14 days. Together with our Airway Epithelial Cell Growth Medium and ALI pre-screened HBEpC, the ALI-Airway medium constitute a standardized system to facilitate reproducible and physiologically relevant respiratory research.

Advantages:

• Minimize lot-to-lot variation by optimized BPE-free and serum-free formulation
• Stable and uniform TEER values of > 500 Ω*cm² until at least 14 days of culture
• ALI pre-screened HBEpC available on request, optionally from HLA-typed and COPD/Asthma donors
• Barrier-forming bronchial epithelial cells will be kept in a healthy status with a cell viability > 70 %

Components:

• 1x Basal Medium, 500ml
• 1x SupplementMix for 500ml

Adding the SupplementMix to the Basal Medium results in complete Growth Medium.