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Primary Cancer Culture System
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A complete defined and animal-component free cell culture solution designed for the selective culture of malignant cells derived from primary tumors or patient-derived xenografts.

Unit 250 ml
Cat.No.
C-28081
$973.00
Currently In stock

Key benefits:

  • Perform up to five CSC isolations with one medium kit
  • Gain access to pure and stable CSC cultures from primary tumors, patient-derived xenografts (PDX), and established cell lines
  • Enables long-term growth support and multiple passaging
  • Defined and animal component-free formulation

Components:

In vivo tumors consist of a heterogeneous population of cancer cell types that include tumor-initiating slow-growing cancer stem cells, fast-growing aggressive cancer cells, tumor-associated cancer cells, immune cells, and cancer stem cells (CSCs). Unlike some other cell types, CSCs have distinct properties such as aberrant metabolism, anoikis resistance, and differentiation ability. And while CSCs only make up for a very small fraction of the tumor mass, they initiate and drive tumor development and, in most cases, trigger metastasis and relapse – making them a promising therapeutic target.

CSCs display a slow proliferation compared to other cancer and non-cancer cell types within the tumor mass and so are easily overgrown by other cell types. In biopsies, for example, CSCs are often overgrown by fibroblasts or aggressive cancer cells, neither of which can induce tumors. In cases like this, long-term cultivation and isolation of CSCs become difficult or impossible.

To achieve the best conditions and avoid CSCs being overgrown, it’s important to take their distinct properties into account and use media developed specifically for their culture and maintenance.

Our Primary Cancer Culture System (PCCS) is a part of the cancer media toolbox, a complete cell culture solution for the isolation and long-term culture of cancer cells. The PCCS is ideal for CSCs from human primary tumor biopsies, patient-derived xenografts (PDX), or commercially available cancer cell lines. The PCCS is flexible enough to be used for most cancer types.

The PCCS is more than a medium: designed to specifically isolate and maintain CSC, the PCCS supports the aberrant metabolic traits of malignant cells. The PCCS is therefore a functional medium. As this principle is not tissue-specific, the PCCS can be used to isolate CSCs from most tumor types.

Primary Cancer Culture System

Due to the PCCS specificity, other cancer cell types and stroma cells are progressively depleted during cultivation. This process usually takes a few to several weeks depending on the intrinsic growth rate of the CSC population within the sample.

The PCCS is highly standardized thanks to its defined and animal component-free (D-ACF) formulation, which ensures the lowest possible lot-to-lot variation and standardized cell culture conditions.

Finally, the PCCS can also be used for other applications, such as enriching cell lines with CSCs or depleting stromal cells and other non-cancerous cells from already established cancer cell cultures and tumor biopsies to identify CSC biomarkers.

Please note: Due to production reasons, we have changed the catalogue number on the basal medium bottle label by the addition of “B”. Therefore, the catalogue number on the basal medium bottle differs from that of the complete Primary Cancer Culture System. This change only affects the label, the volume and formulation remain the same.

Image
Figure 1.Overview of the selective isolation of primary cancer cells from human tumor samples using the Primary Cancer Culture System. The PromoCell system makes it possible to reliably deplete non-malignant cells from the cell culture while supporting the maintenance of cancer cells and malignant cell heterogeneity. The optional establishment of cancer cell lines is indicated.
Image
Figure 2.Primary culture derived from a squamous cell carcinoma featuring an adherent growth pattern. The upper right inset reveals cellular heterogeneity at a greater magnification.
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Figure 3.Primary culture derived from a low-grade small cell lung cancer featuring a suspension growth pattern with 3D sphere formation.

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