Citations
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Human Small Airway Epithelial Cells (HSAEpC)
Primary Human Small Airway Epithelial Cells (HSAEpC) are isolated from the distal portion of the human respiratory tract in the 1 mm bronchiole area. They stain positive for cytokeratin.
The distal respiratory tract mainly consists of pulmonary alveoli, which are spherical outcroppings of the respiratory bronchioles and are the primary sites of gas exchange with the blood. HSAEpC are qualified for functional studies to investigate disorders such as asthma or pulmonary inflammation.
- Request our GMP grade cell culture media for endothelial cells.
- Our HSAEpC are now also available from HLA-typed and COPD/Asthma donors.
Recommended plating density | 10000 - 15000 cells per cm2 |
Passage after thawing | P2 |
Tested markers | Cytokeratin positive |
Guaranteed population doubling | > 15 |
Short protocol:
- Trypsinize the cells as usual
- Centrifuge and resuspend in suitable cold freezing medium at a density of 1-4 x 106 cells/ml
- Cool down the cells slowly to -80°C (approx. -1°C per min). We recommend to use "Mr. Frosty" from Nalge or "CoolCell" from Biocision; which both provide gradual and controlled cooling rates when placed in a -80°C freezer overnight.
- Transfer the vials into liquid nitrogen for long-term storage
- Mesenchymal Stem Cells (C-12974/C-12971/C-12977) need Fibronectin-coating when grown in PromoCell MSC Growth Medium XF (C-28019) and when differentiated in MSC Neurogenic (C-28015); Adipogenic (C-28016); or Osteogenic (C-28013) Differentiation Media.
- Human monocyte-derived macrophages (C-12914/C-12916/C-12915/C-12917) must be seeded into Fibronectin-coated culture vessels in combination with PromoCell's M1- and M2-Generation Media XF (C-28055; C-28056).
- For efficient induction of osteoblast mineralization with PromoCell's Osteoblast Mineralization Medium (C-27020); the TC plates should be pre-coated with collagen type I.
- Liquid phase storage provides a consistent temperature of -196°C; a longer holding time and a greater vial capacity but involves the risk of contamination issues.
- Storage in the gas phase is very safe with respect to contaminations but the holding time of the cells is shorter and the vial capacity is reduced.
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HLA-Typed Human Primary CellsBrochures Go To Resource
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Tools for Respiratory ResearchApplication Notes Go To Resource
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Human Respiratory Cell TypesBrochures Go To Resource
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Cell Disease ModelsBrochures Go To Resource
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HLA-Typed Human Primary CellsBrochures Go To Resource
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The human leukocyte antigen (HLA) complex - relevance of HLA to cancer immunotherapyPosters Go To Resource
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HLA-Typed Primary Cells: The advantages of working with HLA in drug development researchVideos Go To Resource
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The importance of HLA for the development of cell therapiesWhite Papers Go To Resource
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Thawing frozen cells – detailed protocol for human primary cellsVideos Go To Resource
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Generation of Human Airway Organoids from Primary CellsApplication Notes Go To Resource
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