Application Notes
Items 13-24 of 39 Results
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Generation of human airway organoids from primary cells
This Application Note describes the generation of functional 3D airway organoids with apical-out or inward-oriented cilia formation based on Primary Human Bronchial Epithelial Cells (HBEpC) as an in vitro model for high-throughput drug screening or large-scale virus-host interaction studies.
Application Notes
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Development of a high throughput 3D in vitro wound healing model
Studying wound healing is a challenge, however the ability to quantifying the difference between 3D skin models is now possible.
Application Notes
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Air-Liquid Interface Culture System for standardized respiratory research
Generation of a stable and functional 3D human airway model with primary human bronchial epithelial cells using the PromoCell Air-Liquid Interface Culture System.
Application Notes
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Isolation of Tumor Associated Macrophages (TAM) from Fresh Tumor Tissue
Tumor-associated macrophages (TAM) represent an important constituent of the tumor microenvironment (TME).
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Determination of the Tumorsphere Formation Efficiency (TFE)
Determine the tumorsphere formation efficiency using an assay to quantify the ability of single cells to form spheres. Our defined and xeno-free 3D Tumorsphere Medium enables you to cultivate cancer stem cells.
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Tumorsphere culture of cancer stem cells
Find out how to cultivate cancer stem cells in tumorspheres using our defined and xeno-free 3D Tumorsphere Medium. This enables you to characterize such cells and study their malignancy.
Application Notes
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Differentiation of M1- or M2-Macrophages from PBMC/Monocytes
Here we describe how the presence of PromoCell differentiation media encourages in vitro differentiation of mononcytes to macrophages. These then show either an M1-like or an M2-like polarization.
Application Notes
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Generation of monocyte-derived Dendritic Cells (moDCs)
We offer a step-by-step protocol for the in vitro maturation of human monocytes into immature monocyte-derived dendritic cells (moDCs), as well as into fully mature moDCs, using PromoCell media.
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Standardized culture of assay-ready and fully functional human primary macrophages
Cryopreserved human macrophages are now available as a reliable source of standardized cells, and our ready-to-use format provides full experimental flexibility. With this easy-to-handle application, your macrophage research will be more efficient.
Application Notes
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Mammosphere culture of human breast cancer cells
PromoCell 3D Tumorsphere Medium XF allows robust 3D culture even when using breast-cancer cell lines. Mammospheres are now regarded as highly valuable in vitro models for studies on tumor formation and metastasis.
Application Notes
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Neurosphere culture of brain tumor stem cells
Using 3D cultures of brain cancer cells as neurospheres in serum-free conditions maintains the properties of primary tumors. In vitro models with the 3D Tumorsphere Medium XF are an essential prerequisite for research aimed at identifying ways to eradicate brain tumor cancer stem cells.
Application Notes
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Endothelial cell transmigration and invasion assay
Angiogenesis, the development of blood vessels from existing ones, is an essential process. Common in vitro models are the transwell migration assay and the cell invasion assay.
Application Notes