Is it necessary to use the PromoCell DetachKit when subculturing normal human cells?

We recommend using the PromoCell DetachKit (C‑41200) for subculturing PromoCell normal human cells.
The DetachKit was specifically designed for the safe and efficient detachment of primary human cells during routine subculture and consists of three ready‑to‑use components:

• HEPES BSS (HEPES‑buffered Saline Solution)
• Trypsin/EDTA Solution
• TNS (Trypsin Neutralizing Solution)

Important note
Many PromoCell culture media contain low serum levels or no serum at all. These media are not suitable for inactivating trypsin during subculture.
The Trypsin Neutralizing Solution included in the DetachKit ensures reliable and controlled trypsin inactivation under serum‑free or low‑serum conditions.

Alternative detachment method
As an alternative, PromoCell Accutase Solution may be used for certain cell types.
Please refer to the Manual of the respective cell type for guidance on whether Accutase is suitable and recommended.