Is it necessary to use the PromoCell DetachKit when subculturing PromoCell Normal Human Cells?

We recommend to use the DetachKit when subculturing our Normal Human Cells. It contains a HepesBSS washing buffer, trypsin 0.04% / EDTA 0.03% solution, and TNS, a trypsin inhibitor from soybean. Please note: Many of our culture media have low serum content or no serum at all. These media are not suitable to inactivate trypsin during subculture.