Is it necessary to use the PromoCell DetachKit when subculturing PromoCell Normal Human Cells?

We recommend using the DetachKit (C-41200) for subculturing our normal human cells. 

It was designed for the safe and efficient detachment of primary human cells in routine subculturing and consists of 3 components: HEPES BSS (HEPES buffered Balanced Salt Solution), Trypsin/EDTA Solution and TNS (Trypsin Neutralizing Solution). All 3 components are ready-to-use.

Please note: Many of our culture media have low serum content or no serum at all. These media are not suitable for inactivating trypsin during subculture.