Which 96‑well U‑bottom suspension plates does PromoCell recommend for chondrogenic differentiation of hMSC?
For chondrogenic differentiation of human mesenchymal stem cells (hMSC), it is essential that the cells do not adhere to the culture surface.
During differentiation, hMSCs aggregate and form spheroids that float freely in the medium. Therefore:
During differentiation, hMSCs aggregate and form spheroids that float freely in the medium. Therefore:
- No specific plate brand is required
- Any 96‑well U‑bottom plate that is suitable for suspension culture can be used
- The critical requirement is the U‑bottom geometry, which supports spheroid formation and prevents cell attachment
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