The trypsin activity of C‑41000 is now reported in BAEE units. Has the product changed?
No. C‑41000 remains a ready-to-use Trypsin/EDTA solution and should be used as before.
The change from reporting trypsin concentration in % to reporting enzyme activity in BAEE units reflects a more precise way of characterizing trypsin performance and does not require any changes to standard cell culture protocols.
BAEE (Nα‑benzoyl‑L‑arginine ethyl ester) is a synthetic substrate that is commonly used to determine trypsin activity. The BAEE value indicates how efficiently the enzyme cleaves this substrate under defined conditions.
Unlike enzyme concentration, which does not always reflect actual performance, enzyme activity directly measures the functional effectiveness of trypsin. Reporting activity in BAEE units therefore helps ensure consistent and reproducible cell detachment performance and improves batch‑to‑batch comparability.
Please note: The concentration of EDTA has not changed and continues to be specified as a percentage (0.03% w/v).
The change from reporting trypsin concentration in % to reporting enzyme activity in BAEE units reflects a more precise way of characterizing trypsin performance and does not require any changes to standard cell culture protocols.
BAEE (Nα‑benzoyl‑L‑arginine ethyl ester) is a synthetic substrate that is commonly used to determine trypsin activity. The BAEE value indicates how efficiently the enzyme cleaves this substrate under defined conditions.
Unlike enzyme concentration, which does not always reflect actual performance, enzyme activity directly measures the functional effectiveness of trypsin. Reporting activity in BAEE units therefore helps ensure consistent and reproducible cell detachment performance and improves batch‑to‑batch comparability.
Please note: The concentration of EDTA has not changed and continues to be specified as a percentage (0.03% w/v).
