This application note describes a method for easy 3D organoid culture of Primary Human Dermal Fibroblasts (NHDF) and MCF-7 cells in our Cancer Cell Line Medium XF.
Use this protocol to generate cancer organoids easily and reliably. This uses normal human primary dermal fibroblasts (NHDFs) co-cultured with MCF-7 cells but also serves as a flexible template for other cell types. Using Cancer Cell Line Medium XF, it also eliminates undefined components, such as fetal calf serum, extracts, or hydrolysates.
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Learn more about the specific applications of our cancer cell media – from establishing cancer cell lines to developing 3D tumor model or building screening assays.
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