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Is there a difference in the percentage of differentiated dendritic cells when starting from cryopreserved versus fresh monocytes?

No significant difference is observed in the percentage of monocytes that differentiate into dendritic cells (DCs) when comparing freshly isolated and cryopreserved monocytes when using PromoCell DC Generation Medium (C‑28050).
However, there is an important practical difference to consider:
  • Higher initial cell loss with cryopreserved monocytes compared to when fresh monocytes are used.
  • Impact on final number of differentiated cells
As a result, while the differentiation efficiency itself is comparable, the absolute number of differentiated DCs obtained at the end of the culture will usually be higher when starting from fresh monocytes.

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PromoCell Human CD14+ Monocytes (hMoCD14+-PB)
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Human CD14+ Monocytes isolated from peripheral blood (hMoCD14+-PB) of single donors.
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DC Generation Medium (Ready-to-use)
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Cell culture medium for the generation of dendritic cells from peripheral blood monocytes.
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