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Is it possible to thaw and re‑freeze the Cytokine Mix M1 (C‑39894) and M2 (C‑39895)?

No. The Cytokine Mix M1 and Cytokine Mix M2 should not be subjected to additional freeze-thaw cycles.
Repeated freezing and thawing can lead to degradation and loss of biological activity of the cytokines, which may negatively affect macrophage polarization and experimental reproducibility.
To ensure optimal performance, the cytokine mixes should be thawed only once and used immediately.

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