Is it possible to differentiate M1 macrophages from PBMCs in 96‑well plates?

Yes, differentiation of M1 macrophages from PBMCs in 96‑well plates is possible.
At PromoCell, this setup has not been formally validated internally. However, multiple users have reported successful differentiation of mononuclear cells into M1 macrophages using a 96‑well plate format.
 
User‑reported conditions
Based on customer feedback:

• Plating density: Approximately 1 × 10⁶ PBMCs per well, without prior determination of the monocyte content, has been reported to work well.
• Plate format: Standard 96‑well tissue culture plates, coated with collagen type I
• Working volume: Typically 100 µl per well

Under these conditions, users observed efficient differentiation of mononuclear cells into macrophages in the 96‑well format.
The standard protocol for the differentiation of macrophages from PBMC is provided in the corresponding PromoCell Application Note.