Is Cryo‑SFM Plus suitable for the cryopreservation of neurospheres?

Q: Is Cryo‑SFM Plus suitable for the cryopreservation of neurospheres?

PromoCell has successfully cryopreserved intact tumorspheres using Cryo‑SFM Plus . Since neurospheres and tumorspheres are both three-dimensional cellular structures, Cryo‑SFM Plus is expected to be suitable for neurosphere cryopreservation as well. Please note : Neurospheres have not been formally tested with Cryo‑SFM Plus. Therefore, we recommend validating the freezing and thawing conditions for your specific application. For optimal results, neurospheres should be: Healthy and viable prior to freezing Preferably 100-200 µm in diameter No larger than approximately 200-300 µm in diameter This size range helps ensure adequate cryoprotectant penetration and efficient post-thaw recovery.

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Accepted Answer

PromoCell has successfully cryopreserved intact tumorspheres using Cryo‑SFM Plus. Since neurospheres and tumorspheres are both three-dimensional cellular structures, Cryo‑SFM Plus is expected to be suitable for neurosphere cryopreservation as well.

Please note: Neurospheres have not been formally tested with Cryo‑SFM Plus. Therefore, we recommend validating the freezing and thawing conditions for your specific application.
For optimal results, neurospheres should be:

Healthy and viable prior to freezing
Preferably 100-200 µm in diameter
No larger than approximately 200-300 µm in diameter

This size range helps ensure adequate cryoprotectant penetration and efficient post-thaw recovery.

Quote Summary

Is Cryo‑SFM Plus suitable for the cryopreservation of neurospheres?

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