Powered by BiozHuman Osteoblasts (HOB)
Primary Human Osteoblasts isolated from femoral trabecular bone tissue from the knee or hip joint region.
Unit
500,000 cells
Cat.No.
C-12720
$1,010.00
Currently In stock
Primary Human Osteoblasts (HOB) are isolated from femoral trabecular bone tissue from the knee or hip joint region (lot specific source information is available on request). They stain positive for alkaline phosphatase and can be mineralized using our Osteoblast Mineralization Medium.
Osteoblasts are of mesenchymal origin and are mainly responsible for bone formation and remodeling. In vivo, they produce a collagen type I rich extracellular matrix called the osteoid.
During their development, osteoblasts undergo a maturation process called osteogenesis. When they become encased in the bone matrix, they stop proliferating and become osteocytes.
- Our HOB are now also available from HLA-typed donors.
| Recommended plating density | 10000 - 20000 cells per cm2 |
| Passage after thawing | P2 |
| Tested markers | Alkaline phosphatase positive, Mineralization tested |
| Guaranteed population doubling | > 10 |
Figure 1.Human Osteoblast culture in phase contrast.
Figure 2.Alkaline phosphatase activity in HOB stained with BCIP/NBT.
Figure 3.Mineralized HOB. Calcium deposit stained with Alizarin Red S.
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