Human Mononuclear Cells (hMNC)
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Mononuclear Cells (MNC) represent the lymphocyte and monocyte fraction isolated from umbilical cord blood or peripheral blood. They are prepared by optimized low-density gradient centrifugation, which effectively reduces the granulocyte content. Red blood cells are gently but thoroughly depleted by proprietary techniques instead of osmotic lysis. This results in ultra-pure mononuclear cells with superior viability and unchanged biological function. In addition, the cells do not clump after thawing, allowing for easy handling and reproducible results.
The MNC can be used directly for experiments or as a starting material for the isolation of several cell types, e.g., hematopoietic and endothelial progenitors, monocytes, and lymphocyte subpopulations.
Please note: Cryopreserved human mononuclear cells are not suitable for the in vitro generation of macrophages using our Macrophage Generation Media XF. Always use freshly isolated human mononuclear cells or human monocytes for this application.
- Our hMNC are now also available from HLA-typed donors.
| Recommended plating density | 1,000,000 cells per ml |
| Tested markers | Scatter (flow cytometry) for cell size and granularity identify Lymphocytes, Monocytes and Granulocytes in hMNC-CB |
| Recommended culture media | C-28030 |
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