Human Mesenchymal Stem Cells (hMSC)

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PromoCell Human Mesenchymal Stem Cells from Bone Marrow (hMSC-BM)

Instruction manual SDS

Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.

Note: Umbilical cord matrix hMSC are currently not available.

Human Mesenchymal Stem Cells (hMSC)

Cat No IV-4490

Unit

943.00$

Ready to ship

Product description

Mesenchymal Stem Cells (MSC), also termed Mesenchymal Stromal Cells, are multipotent cells that can differentiate into a variety of cell types and have the capacity for self renewal. MSC have been shown to differentiate in vitro or in vivo into adipocytes, chondrocytes, osteoblasts, myocytes, neurons, hepatocytes, and pancreatic islet cells. Optimized standard and GMP grade media are available to support both the growth of MSC and their differentiation into several different lineages. Recent experiments suggest that differentiation capabilities into diverse cell types vary between MSC of different origin.

Our hMSC are harvested from normal human adipose tissue, bone marrow, and umbilical cord matrix (Wharton’s jelly) of individual donors.

The cells are tested for their ability to differentiate in vitro into adipocytes, chondrocytes, and osteoblasts. Our hMSC show a verified marker expression profile that complies with ISCT* recommendations, providing well characterized cells.

Request our GMP grade cell culture media for mesenchymal stem cells.
Our hMSC are now also available from HLA-typed donors.

Recommended plating density	4,000 cells per cm²
Passage after thawing	P2
Tested markers	Differentiation capacity to adipogenic, osteogenic and chondrognic cells tested, CD105 positive, CD73 positive, CD90 positive, CD45 negative, CD34 negative, CD14 negative, CD19 negative, HLA-DR negative
Guaranteed population doubling	> 10
Recommended culture media+	C-28009, C-28019

*ISCT (International Society for Cellular Therapy) Cytotherapy (2006) Vol. 8, No. 4, 315-317

+The catalog numbers in this table are for media in ready-to-use packaging.

Data & figures

Figure 1. Human Mesenchymal Stem Cell Culture isolated from bone marrow in MSC Growth Medium in phase contrast.

Figure 2. Human Mesenchymal Stem Cell Culture isolated from bone marrow in MSC Growth Medium XF in phase contrast.

Figure 3. Human Mesenchymal Stem Cell Culture isolated from adipose tissue in MSC Growth Medium in phase contrast.

Figure 4. hMSC-BM after in vitro differentiation into adipocytes using PromoCell Mesenchymal Stem Cell Adipogenic Differentiation Medium.

Figure 5. hMSC-BM spheroid after in vitro differentiation into cartilage using our Mesenchymal Stem Cell Chondrogenic Differentiation Medium (Alcian Blue staining).

Figure 6. hMSC-BM after in vitro differentiation into osteoblasts using our Mesenchymal Stem Cell Osteogenic Differentiation Medium (Alizarin Red S staining).

Figure 7. hMSC-BM after in vitro differentiation into neuronal cells using our Mesenchymal Stem Cell Neurogenic Differentiation Medium.

Technical library

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