How should I best freeze PromoCell normal human cells?
For optimal post‑thaw recovery, please follow the protocol below when freezing normal human cells.
Cell freezing protocol
1. Detach the cells using your standard detachment procedure.
2. Count the cells and ensure a viability greater than 70% before freezing.
3. Centrifuge the cells and resuspend them in a suitable freezing medium at a density of 0.1–10 × 10⁶ cells/ml.
4. Aliquot the cell suspension into cryovials.
5. Cool the cells slowly to −80°C at an approximate rate of −1°C per minute.
6. Transfer the frozen vials to liquid nitrogen for long‑term storage.
Cell freezing protocol
1. Detach the cells using your standard detachment procedure.
2. Count the cells and ensure a viability greater than 70% before freezing.
3. Centrifuge the cells and resuspend them in a suitable freezing medium at a density of 0.1–10 × 10⁶ cells/ml.
- We recommend Cryo‑SFM Plus, a defined, animal‑component‑free and protein‑free cryopreservation medium.
- The freezing medium should be pre‑cooled to 4°C. Do not exceed room temperature.
4. Aliquot the cell suspension into cryovials.
5. Cool the cells slowly to −80°C at an approximate rate of −1°C per minute.
- We recommend using a controlled‑rate freezing container such as Mr. Frosty™ (Nalgene) and placing it in a −80°C freezer overnight.
6. Transfer the frozen vials to liquid nitrogen for long‑term storage.
