For how long can monocyte‑derived dendritic cells be maintained in culture after differentiation is completed (day 7/8)?
Based on PromoCell’s experience, monocyte‑derived dendritic cells (DCs) can be maintained in culture for approximately 3 up to a maximum of 7 days after completion of differentiation (day 7/8 of the protocol).
Important considerations
Important considerations
- During this post‑differentiation period, cell morphology will gradually change
- Over time, the cells may appear increasingly degenerate
- Functional and phenotypic stability may therefore decline with prolonged culture
Products relevant to this topic
DC Generation Medium (Ready-to-use)
Cat No
C-28050
Cell culture medium for the generation of dendritic cells from peripheral blood monocytes.
634.00$
DC Generation Medium XF (Ready-to-use)
Cat No
C-28052
Serum-free and xeno-free medium for the generation of dendritic cells from peripheral blood monocytes. The Base Medium XF does not include cytokines.
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