Do I have to use coated tissue culture vessels for culturing PromoCell Normal Human Cells?
It is not necessary to use coated flasks for (most of) our Normal Human Cells but it can be done. As coating with extracellular matrix proteins can affect cellular metabolism, it is recommended to use the same coating material for a complete set of experiments.
Cells that need to be grown on coated dishes:
- Mesenchymal Stem Cells (C-12974/C-12971/C-12977) need FN- or VN-, or Collagen type I-coating (for details, please view the Product Manuals) when grown in PromoCell MSC Growth Medium XF (C-28019) and/or when being differentiated in our MSC Neurogenic (C-28015), Adipogenic (C-28016), or Osteogenic (C-28013) Differentiation Media.
- Human monocyte-derived macrophages (C-12914/C-12916/C-12915/C-12917) must be seeded into Fibronectin- or Vitronectin-coated culture vessels in combination with PromoCell's M1- and M2-Generation Media XF (C-28055, C-28056).
- For efficient induction of osteoblast mineralization with PromoCell's Osteoblast Mineralization Medium (C-27020), the TC plates should be pre-coated with collagen type I.
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Normal Human Epidermal Keratinocytes (NHEK) adult, isolated in Growth Medium 3, pooled
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Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
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