Cryo-SFM Plus - Animal component-free cryopreservation medium for reliable cell storage and recovery
The thaw-freeze cycle is one of the most critical steps in many cell culture workflows. During cryopreservation, freezing and thawing can compromise cell integrity, viability, and post-thaw recovery. Cryo-SFM Plus is a defined, animal component-free and protein-free cryopreservation medium developed to support consistent cell storage and reproducible recovery after thawing.
Nominated for the Scientists' Choice Awards 2025, Cryo-SFM Plus provides a standardized solution for optimal storage of human and animal cells. Explore the available formulations below to view product details and specifications.
Discover Cryo-SFM Plus - A new benchmark in animal component-free cryopreservation
Proven post-thaw recovery and growth
Cryo-SFM Plus has been evaluated in comparative cryopreservation studies using representative primary cells and stem cell models, including primary melanocytes and mesenchymal stem cells (MSCs). These studies demonstrate improved recovery and growth after thawing compared with conventional freezing media.
Improved recovery and growth after thawing
Conventional freezing medium
Cryo-SFM Plus
Figure 1: Primary melanocytes cryopreserved in Cryo-SFM Plus show improved attachment and growth after thawing compared with cells frozen in a conventional freezing medium.
Quantitative post-thaw analysis
Figure 2: Cryo-SFM Plus delivers higher cell yield and faster post-thaw growth compare with a conventional freezing medium.
Key observations
- Primary melanocytes: 36 % faster growth after thawing (lower population doubling time) compared with a conventional freezing medium
- Mesenchymal stem cells (MSCs): 17 % higher cell yield after 72 hours compared with a leading serum-free freezing medium
- Improved cell morphology and attachement after thawing
Defined, protein-free, animal component-free formulation
Cryo-SFM enables consistent cell storage and reliable recovery after thawing.
Key features include:
- Optimal cell yield: Supports high recovery of cells after thawing
- Protein-free and animal component-free: Minimizes risk of contamination or variability in cell cultures
- Innovative antioxidant technology: Preserves viability, attachement, and growth
- Powered by patented technology* combining cryoprotectants with an antioxidant formulation
Suitable for a variety of cell types
We developed Cryo-SFM Plus to enable consistent cryopreservation across a wide range of cell types, including:
- Primary cells (e.g., melanocytes)
- Stem cells (e.g., MSCs, iPSC workflows)
- Immune cells (e.g., PBMCs)
- Established cell lines and cell aggregates (e.g., tumorspheres)
Learn more: Cryopreservation protocol and performance data
Optimize your cell freezing workflows with Cryo-SFM Plus Application Note, featuring detailed experimental data and practical protocol guidance.
Our cryopreservation Application Note includes:
- A detailed freezing and thawing protocol
- Quantitative post-thaw proliferation and viability profiles
- Comparative benchmarking data in sensitive cell types
- Practical guidance for optimizing recovery after thawing
Cryopreservation success starts with the right medium - and the right method.
*Our technology is patented in Germany and pending approval in other countries.
Need help selecting the right freezing medium?
Have questions about cryopreservation protocols, sensitive cell handling, or recovery optimization?
Our scientific support team is here to helpt.