Is it necessary to heat inactivate FCS for use with Normal Human Cells?

Description
The practice of heat inactivation was originally developed when only serum from adult animals was available. Adult serum contains high serum complement which may destroy cells under certain conditions. Heating serum (30 min 56°C) is intended to inactivate the complement. Today serum is often heat-inactivated without any evidence of beneficial effect. When using FCS (fetal calf serum) heat inactivation is not necessary for most cell lines or cell types. PromoCell does not use heat-inactivated FCS for the preparation of their growth media.
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