Typically once seeded how long does it take to grow the chondrocytes to subconfluence? How many doublings will they undergo per passage?

It usually takes 5 to 8 days to grow our Normal Human Chondrocytes (C-12710) to subconfluency. The number of doublings (PDs) they undergo can be calculated from the number of seeded cells and the cell yield at subconfluency. Generally; when HCH are plated with 10;000 cells/cm² they perform between 1.5 and 2 doublings per passage.