Should the skeletal muscle cells be split after adding the Differentiation Medium (C-23061)?

After adding the Skeletal Muscle Cell Differentiation Medium; myoblasts will start to differentiate into myotubes and stop growing. The cells should not be split anymore. It is recommended rather to plate the SkMC into the needed vessels; (e.g. multiwell plates); prior to induction of differentiation and to perform studies directly on differentiated cells. If it is necessary for your tests to detach the myotubes and they are difficult to trypsinze; you can use a "rubber policeman".