How many passages can I perform with PromoCell Normal Human Cells?

PromoCell does not determine the number of passages but instead we calculate the population doublings (PD) that can be performed with the cells. The term passage only describes the process of detachment and replating and does not take into account different split ratios. The optimal split ratio is calculated from the actual cell yield after trypsinisation and the recommended plating density. In most of our cell types; the split ratio is usually between 1:3 and 1:6. Using 1:4 splits (i.e. increasing the growth surface by factor 4 each time); 15 doublings are achieved after 6-8 passages. For recommended plating densities; please view the respective Manual; section "Specifications".