I would like to know how homogenous the MSC population is and if all cells become osteoblasts when grown in MSC Osteogenic Differentiation Medium (C-28013).

Human Mesenchymal Stem Cells have retained the ability to differentiate into a variety of cell types including fat cells; chondrocytes; and osteoblasts. PromoCell supply hMSC from 3 different tissues: bone marrow (hMSC-BM; C-12974); umbilical cord matrix (hMSC-UC; C-12971); and adipose tissue (hMSC-AT; C-12977).

• MSC-BM show very good differentiation into bone cells but also into chondrocytes and fat cells when the respective Differentiation Media is used.
• MSC-UC have a high potential to differentiate into chondrocytes but only weak potential for fat or bone cell differentiation.
• In contrast; MSC-AT differentiate very well into fat and bone cells but only moderately into chondrocytes.

In other words; to obtain a high percentage of bone cells; MSC-BM or MSC-AT are the cells of choice. There are of course lot-to-lot variations and the differentiation will decrease from passage to passage. If you need MSCs with a particularly high osteoblast differentiation capacity; you can call our Technical Customer Service before placing your order so that we can select an appropriate cell lot for you.