Can the size or size distribution of airway organoids be controlled?

Possibly. Airway organoid size and size distribution may be influenced by nutrient availability, particularly within the extracellular matrix (ECM) gel.
A published study suggests that organoid size can be affected by nutrient access in the matrix.
 
Practical recommendations
Based on our experience, the following points can help improve size consistency:
1. Avoid nutrient gradients: 

• Use small, drop‑like BME gel beads
• Combine with relatively high medium volumes
• Change the medium daily, as less frequent changes can result in yellow medium, indicating a pH shift likely caused by increased cellular metabolism

2. Control ECM gelling carefully:
Temperature is critical:

• BME is liquid at 4°C
• BME solidifies at 37°C

Gelling must occur rapidly; otherwise, cells may sink to the bottom of the gel, leading to uneven cell distribution and variable organoid sizes.