Can melanocytes be frozen again after subculture, similar to cell lines?
Upon arrival, PromoCell melanocytes can either be thawed immediately and seeded into a tissue culture vessel or stored in liquid nitrogen until needed.
Re‑freezing after subculture
It is technically possible to re‑freeze normal human cells, including melanocytes. However, PromoCell does not recommend repeated freezing, as each freeze-thaw cycle reduces the cells’ proliferation potential.
Recommendation
If freezing after subculture is required:
Re‑freezing after subculture
It is technically possible to re‑freeze normal human cells, including melanocytes. However, PromoCell does not recommend repeated freezing, as each freeze-thaw cycle reduces the cells’ proliferation potential.
Recommendation
If freezing after subculture is required:
- Use PromoCell Cryo‑SFM Plus (C‑29920) as the freezing medium.
- Freeze the cells at an early passage, as this helps preserve viability and growth potential.
Products relevant to this topic
Normal Human Epidermal Melanocytes (NHEM)
Cat No
C-12400
Primary Human Epidermal Melanocytes isolated in PMA-containing Melanocyte Growth Medium from the epidermis of juvenile foreskin.
651.00$
Normal Human Epidermal Melanocytes (NHEM) adult donor, cultured in M3 Medium
Cat No
C-12413
Primary human epidermal melanocytes isolated in PMA-free Melanocyte Growth Medium M3. Available from juvenile and adult donors.
765.00$
Normal Human Epidermal Melanocytes (NHEM) juvenile foreskin, cultured in M3 Medium
Cat No
C-12422
Primary human epidermal melanocytes isolated in PMA-free Melanocyte Growth Medium M3. Available from juvenile and adult donors.
760.00$
Cryo-SFM Plus, 30 ml
Cat No
C-29920
Defined, animal-component free, and protein-free cryopreservation medium for optimal cell storage.
53.00$