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Can I expand human mesenchymal stem cells (hMSCs) before inducing differentiation?

Yes. PromoCell human mesenchymal stem cells (hMSCs) can be expanded prior to differentiation.
The cells are cryopreserved at the end of the second culture. After thawing and seeding, they are therefore in passage 2 (third culture).
 
For optimal results, we recommend:
  • Inducing differentiation no later than passage 5.
The differentiation potential of hMSCs decreases with increasing population doublings. Consequently, earlier induction of differentiation generally results in higher differentiation efficiency.

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