Are there any differences in the cultivation protocol when cultivating MSCs compared to other cell types?

Yes, there are a few differences:

• We recommend replacing the MSC Growth Medium XF (C-28019) 3-4 h after seeding, as opposed to 16-24 hours after seeding for most other cell types/growth media, including MSC Growth Medium 2.
• IF MSC Growth Medium XF, MSC Neurogenic Differentiation Medium, MSC Adipogenic Differentiation Medium 2, or MSC Osteogenic Differentiation Medium are used, the TC vessels must be pre-coated according to the instruction manual.
• We strongly recommend using Accutase (C-41310) for cell detachment instead of Trypsin. If Trypsin is used, the contact time should not exceed 2 min.