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Melanocyte Growth Medium M3
Serum-free and BPE-free cell culture medium for the cultivation of juvenile and adult human melanocytes. Formulation is PMA-free.
Melanocyte Growth Medium M3 (Ready-to-use)
Our Melanocyte Growth Medium M3 is designed for the isolation and cultivation of human melanocytes from human juvenile and human adult skin in a BPE- and serum-free environment. The medium does not contain PMA (Phorbol Myristate Acetate), which could interfere with some experimental settings. Cells cultured in this medium can be plated directly on tissue culture plastic and no matrix coating is needed. Isolated human juvenile and adult melanocytes show their characteristic dendritic morphology when cultured in this advanced cell culture medium.
Advantages:
- Highest degree of standardization achieved by BPE-free and serum-free formulation
- No adverse effects of PMA (Phorbol Myristate Acetate) on experimental settings
- Each produced media lot is tested for growth promoting activity and characteristic dendritic cell morphology
- Compatible with all available PromoCell Melanocytes (NHEM)
After addition of the SupplementMix or SupplementPack to our basal medium do I have to add FCS you obtain the complete growth medium. No further supplementation with serum or growth factors is required. Please note: Our media do not contain antibiotics. If you wish to use antibiotics you can add penicillin/streptomycin or gentamicin/amphotericin B at standard concentrations. Addition of antibiotics can however reduce the doubling time of the cells up to 30-40%.
The qualitative and quantitative composition of the supplements can be found on our website and in the data sheets of the specialized media. When there is no such information specified; the composition of the supplements is confidential.
Yes, our NHEM.f will also grow in our optimized Melanocyte Growth Medium M3 (C-24310) and can achieve > 15 population doublings.









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