Powered by BiozMelanocyte Growth Medium
Cell culture medium for the cultivation of juvenile human melanocytes. Formulation contains phorbol myristate acetate.
Our Melanocyte Growth Medium is a PMA (Phorbol Myristate Acetate)-containing medium and has been developed for the in vitro cultivation of juvenile melanocytes. Since PMA is a tumor promoting mitogen, it may interfere with some experimental approaches. In these cases, we recommend using the PMA-free Melanocyte Growth Medium M3.
Although all our media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for equine, porcine, murine, and rat melanocytes. View a list of references where this medium was used with other species and cell types.
Components:
- Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix
- Growth Medium Kit: Includes Basal Medium and SupplementPack
- SupplementMix: Contains all media supplements premixed in one vial
- SupplementPack: Contains all media supplements as individual vials
Note: While the supplements of the Melanocyte Growth Medium are serum-free, the Basal Medium is manufactured with 0.2% serum.
| Media supplement | Final supplement concentration |
|---|---|
| Bovine Pituitary Extract | 0.004 ml/ml |
| Basic Fibroblast Growth Factor (recombinant human) | 1 ng/ml |
| Insulin (recombinant human) | 5 μg/ml |
| Hydrocortisone | 0.5 μg/ml |
| Phorbol Myristate Acetate | 10 ng/ml |
Please note: Due to production reasons, we have changed the label on the basal medium bottle of our Ready-to-use growth media from “Growth Medium” to “Basal Medium.” This change only affects the label; the volume and formulation remain the same. Please note that the catalogue number on the basal medium bottle differs from that of the complete Ready-to-use growth medium.
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Normal Human Epidermal Melanocytes (NHEM)
Primary Human Epidermal Melanocytes isolated in PMA-containing Melanocyte Growth Medium from the epidermis of juvenile foreskin.