Is surface coating recommended for calcification assays using vascular smooth muscle cells?
Based on feedback received from users, coating the culture vessel with Fibronectin or Poly‑L‑Lysine is recommended for calcification studies with vascular smooth muscle cells.
Users have reported successful calcification of PromoCell Human Aortic Smooth Muscle Cells (HAoSMC) cultured on Fibronectin‑ or Poly‑L‑Lysine‑coated culture vessels and treated with Osteoblast Mineralization Medium (C-27020) in a 21‑day calcification assay.
In addition, successful calcification has been reported for Human Coronary Artery Smooth Muscle Cells (HCASMC) cultured on Fibronectin‑coated culture vessels.
Please note: These observations are based on user feedback and have not been independently validated by PromoCell.
Users have reported successful calcification of PromoCell Human Aortic Smooth Muscle Cells (HAoSMC) cultured on Fibronectin‑ or Poly‑L‑Lysine‑coated culture vessels and treated with Osteoblast Mineralization Medium (C-27020) in a 21‑day calcification assay.
In addition, successful calcification has been reported for Human Coronary Artery Smooth Muscle Cells (HCASMC) cultured on Fibronectin‑coated culture vessels.
Please note: These observations are based on user feedback and have not been independently validated by PromoCell.
Products relevant to this topic
Human Coronary Artery Smooth Muscle Cells (HCASMC)
Cat No
C-12511
Human Smooth Muscle Cells isolated from the human coronary arteries.
Human Aortic Smooth Muscle Cells (HAoSMC)
Cat No
C-12533
Primary Human Aortic Smooth Muscle Cells isolated from the human aorta.
Smooth Muscle Cell Growth Medium 2 (Ready-to-use)
Cat No
C-22062
Low-serum cell culture medium for smooth muscle cells from blood vessels and hollow organs.
Osteoblast Mineralization Medium
Cat No
C-27020
Mineralization medium for induction of mineralization in human osteoblast cultures.