Is surface coating recommended for calcification assays using vascular smooth muscle cells?
Based on feedback received from users, coating the culture vessel with Fibronectin or Poly‑L‑Lysine is recommended for calcification studies with vascular smooth muscle cells. Users have reported successful calcification of PromoCell Human Aortic Smooth Muscle Cells (HAoSMC) cultured on Fibronectin‑ or Poly‑L‑Lysine‑coated culture vessels and treated with Osteoblast Mineralization Medium (C-27020) in a 21‑day calcification assay. In addition, successful calcification has been reported for Human Coronary Artery Smooth Muscle Cells (HCASMC) cultured on Fibronectin‑coated culture vessels.
Please note: These observations are based on user feedback and have not been independently validated by PromoCell.
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