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Macrophage Base Medium XF
User-customizable, serum-free and xeno-free medium for generation of macrophages from fresh monocytes. Medium does not include cytokines.
Our Macrophage Base Medium XF is the user-customizable version of the Macrophage Generation Media XF and does not include cytokines. It comes without cytokines as a universally applicable MDM culture system featuring a fully user-customizable macrophage differentiation and activation process.
Although all our media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine macrophages. View a list of references where this medium was used with other species and cell types.
Components:
- Basal Medium (250 ml)
- SupplementMix (for 250 ml)
Note: Requires additional supplementation with M-CSF / GM-CSF.
More details for differentiation factor/activator combinations and recommended concentrations are available in our application note: Monocyte-derived macrophages.

Usually we do not recommend specific plasticware but we have found that with PromoCell’s Dendritic Cell and Macrophage Generation Media choice of plasticware can have a lot of an influence. For our Dendritic Cell Generation Media we recommend to use tissue culture vessels from BD FalconTM.
After addition of the SupplementMix or SupplementPack to our basal medium do I have to add FCS you obtain the complete growth medium. No further supplementation with serum or growth factors is required. Please note: Our media do not contain antibiotics. If you wish to use antibiotics you can add penicillin/streptomycin or gentamicin/amphotericin B at standard concentrations. Addition of antibiotics can however reduce the doubling time of the cells up to 30-40%.
The qualitative and quantitative composition of the supplements can be found on our website and in the data sheets of the specialized media. When there is no such information specified; the composition of the supplements is confidential.








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