Can I use a PromoCell Basal Medium to prepare my own freezing medium?
No, we do not recommend using PromoCell Media to prepare your own freezing medium.
Our Basal Media are highly complex formulations. Freezing can cause certain components to precipitate irreversibly, which may negatively impact cell viability and post-thaw recovery. Since PromoCell has not evaluated or validated its Media for use as a freezing medium base, we cannot provide recommendations regarding their suitability for this purpose.
For cryopreservation, we recommend using either:
Our Basal Media are highly complex formulations. Freezing can cause certain components to precipitate irreversibly, which may negatively impact cell viability and post-thaw recovery. Since PromoCell has not evaluated or validated its Media for use as a freezing medium base, we cannot provide recommendations regarding their suitability for this purpose.
For cryopreservation, we recommend using either:
- A standard freezing medium consisting of 70% DMEM, 20% fetal calf serum (FBS), and 10% DMSO, or
- Cryo-SFM Plus (C-29920), PromoCell's serum-free, animal component-free cryopreservation medium.
Products relevant to this topic
Cryo-SFM Plus, 30 ml
Cat No
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Defined, animal-component free, and protein-free cryopreservation medium for optimal cell storage.
Keratinocyte Growth Medium 2 (Ready-to-use)
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Serum-free cell culture medium for keratinocytes from the epidermis.
Keratinocyte Growth Medium 3
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Serum-free and BPE-free cell culture medium for the cultivation of human keratinocytes from the epidermis of juvenile foreskin or adult skin.
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Serum-free and BPE-free cell culture medium for epithelial cells isolated from large air passages.
Airway Epithelial Cell Growth Medium (Ready-to-use)
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Ready-to-use serum-freecell culture medium for epithelial cells from large air passages.
Small Airway Epithelial Cell Growth Medium (Ready-to-use)
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Serum-free cell culture medium for epithelial cells from the distal respiratory tract.
Air-Liquid Interface Medium
Cat No
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Serum-free and BPE-free cell culture medium for an optimal and standardized culture of human bronchial epithelial cells at the air-liquid interface (ALI).