Can I use a PromoCell Basal Medium to prepare my own freezing medium?

No, we do not recommend using PromoCell Media to prepare your own freezing medium.
Our Basal Media are highly complex formulations. Freezing can cause certain components to precipitate irreversibly, which may negatively impact cell viability and post-thaw recovery. Since PromoCell has not evaluated or validated its Media for use as a freezing medium base, we cannot provide recommendations regarding their suitability for this purpose.
For cryopreservation, we recommend using either:

• A standard freezing medium consisting of 70% DMEM, 20% fetal calf serum (FBS), and 10% DMSO, or
• Cryo-SFM Plus (C-29920), PromoCell's serum-free, animal component-free cryopreservation medium.

When working with low-serum or serum-free cell culture systems, the use of a serum-free freezing medium such as Cryo-SFM Plus is particularly recommended to maintain consistency throughout the cell culture workflow and to avoid exposing cells to high serum concentrations that are not present under normal culture conditions.