Citations
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Human Coronary Artery Smooth Muscle Cells (HCASMC)
Primary Human Coronary Artery Smooth Muscle Cells (HCASMC) are isolated from human coronary arteries and stain positive for smooth muscle α-actin. Coronary arteries supply the heart with blood and obstruction in atherosclerosis causes myocardial infarction.
HCASMC are qualified to study the function of smooth muscle cells in the development of atherosclerotic plaques or in other diseases like hypertension. They are also useful for stent-graft compatibility testing.
- Request our GMP grade cell culture media for smooth muscle cells.
- Our HCASMC are now also available from HLA-typed and Diabetes Type I and Type II donors.
Recommended plating density | 5000 - 10000 cells per cm2 |
Passage after thawing | P2 |
Tested markers | Smooth muscle α-actin positive, CD31 negative |
Guaranteed population doubling | > 15 |
- Right coronary artery
- Left main coronary artery
- Circumflex coronary artery and
- Left anterior descending coronary artery.
Short protocol:
- Trypsinize the cells as usual
- Centrifuge and resuspend in suitable cold freezing medium at a density of 1-4 x 106 cells/ml
- Cool down the cells slowly to -80°C (approx. -1°C per min). We recommend to use "Mr. Frosty" from Nalge or "CoolCell" from Biocision; which both provide gradual and controlled cooling rates when placed in a -80°C freezer overnight.
- Transfer the vials into liquid nitrogen for long-term storage
- Mesenchymal Stem Cells (C-12974/C-12971/C-12977) need Fibronectin-coating when grown in PromoCell MSC Growth Medium XF (C-28019) and when differentiated in MSC Neurogenic (C-28015); Adipogenic (C-28016); or Osteogenic (C-28013) Differentiation Media.
- Human monocyte-derived macrophages (C-12914/C-12916/C-12915/C-12917) must be seeded into Fibronectin-coated culture vessels in combination with PromoCell's M1- and M2-Generation Media XF (C-28055; C-28056).
- For efficient induction of osteoblast mineralization with PromoCell's Osteoblast Mineralization Medium (C-27020); the TC plates should be pre-coated with collagen type I.
- Liquid phase storage provides a consistent temperature of -196°C; a longer holding time and a greater vial capacity but involves the risk of contamination issues.
- Storage in the gas phase is very safe with respect to contaminations but the holding time of the cells is shorter and the vial capacity is reduced.
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