Which medium should I use to differentiate hMSC into bone cells and maintain them after differentiation?

Osteogenic differentiation of human mesenchymal stem cells (hMSC) using PromoCell MSC Osteogenic Differentiation Medium typically takes approximately 14 days.
A detailed, validated protocol is provided in the Product Manual and the corresponding Application Note.
 
Recommended culture conditions

• Use collagen‑ or fibronectin‑coated tissue culture plates.
• Change the medium every third day during differentiation.

Maintenance after differentiation
After approximately 2 weeks, when osteogenic differentiation is complete, the differentiated bone cells tend to detach from uncoated plastic surfaces.
For this reason:

• Functional assays and analyses should be performed promptly after differentiation.
• Cells should be maintained in MSC Osteogenic Differentiation Medium until the end of the experiment.