Which medium should I use to differentiate hMSC into bone cells and maintain them after differentiation?
Osteogenic differentiation of human mesenchymal stem cells (hMSC) using PromoCell MSC Osteogenic Differentiation Medium typically takes approximately 14 days.
A detailed, validated protocol is provided in the Product Manual and the corresponding Application Note.
Recommended culture conditions
After approximately 2 weeks, when osteogenic differentiation is complete, the differentiated bone cells tend to detach from uncoated plastic surfaces.
For this reason:
A detailed, validated protocol is provided in the Product Manual and the corresponding Application Note.
Recommended culture conditions
- Use collagen‑ or fibronectin‑coated tissue culture plates.
- Change the medium every third day during differentiation.
After approximately 2 weeks, when osteogenic differentiation is complete, the differentiated bone cells tend to detach from uncoated plastic surfaces.
For this reason:
- Functional assays and analyses should be performed promptly after differentiation.
- Cells should be maintained in MSC Osteogenic Differentiation Medium until the end of the experiment.
Products relevant to this topic
Human Mesenchymal Stem Cells from Umbilical Cord Matrix (hMSC-UC)
Cat No
C-12971
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Human Mesenchymal Stem Cells from Bone Marrow (hMSC-BM)
Cat No
C-12974
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Human Mesenchymal Stem Cells from Adipose Tissue (hMSC-AT)
Cat No
C-12977
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Mesenchymal Stem Cell Growth Medium 2
Cat No
C-28009
Low-serum cell growth medium for mesenchymal stems cells.
Mesenchymal Stem Cell Osteogenic Differentiation Medium
Cat No
C-28013
Cell culture medium for differentiation of mesenchymal cells into osteogenic lineages.