What is the time frame for the differentiation of HWP? Can PromoCell provide a differentiation protocol?

Yes. PromoCell provides a validated differentiation protocol for Human White Preadipocytes (HWP), which is described in the Instruction Manual of the respective media.

Recommended differentiation workflow and time frame

• Expansion phase
  • Grow HWP in Preadipocyte Growth Medium (C‑27410) until complete confluence is reached.
  • This phase typically takes approximately 1 week.
  • Change medium 2–3 times per week.
• Induction of differentiation
  • Aspirate the Growth Medium.
  • Add Preadipocyte Differentiation Medium (C‑27437) for 72 hours.
  • Aspirate the Differentiation Medium and switch to Adipocyte Nutrition Medium (C‑27438).
• Differentiation and maturation
  • Cells begin to accumulate lipid droplets, which can be visualized by light microscopy.
  • The differentiation process usually takes 1-2 weeks.
  • Change medium 2-3 times per week.
  • Differentiated adipocytes can be maintained in Nutrition Medium for up to 4 weeks. However, cell lysis and detachment commonly occur after approximately 3 weeks.

Important note
For optimal differentiation efficiency, we strongly recommend performing differentiation experiments at low population doubling (PD) numbers, ideally below PD 4-5, corresponding to a maximum of one passage after thawing.