What is the time frame for the differentiation of HWP? Can PromoCell provide a differentiation protocol?

Induction of differentiation:

• Grow the cells in Preadipocyte Growth Medium (C-27410) until they reach complete confluence; this roughly takes 1 week. Change medium 2-3x per week.
• Aspirate the Growth Medium; add Preadipocyte Differentiation Medium (C-27437) for 72 h
• Aspirate the Differentiation Medium, add Adipocyte Nutrition Medium (C-27438).

The cells will now start to accumulate droplets of lipids which can be visualized under the microscope. The process usually takes 1-2 weeks. Change medium 2-3x per week. It is possible to maintain the differentiated adipocytes in Nutrition Medium for up to 4 weeks. However, the cells tend to lyse and detach after about 3 weeks.

Please note: We recommend to perform differentiation experiments at population doubling numbers lower than 4-5 (a maximum of 1 passage after thawing) in order to obtain a high differentiation level of the culture.