Normal Human Epidermal Melanocytes M3 (NHEM M3)

Primary Normal Human Epidermal Melanocytes M3 (NHEM M3) are isolated from the epidermis of juvenile foreskin or adult skin from different locations including face, breasts, abdomen, and thighs.

Melanocytes are located in the stratum basale, but branch out between the keratinocytes in suprabasal layers. About 5-10% of the cells in the epidermis are melanocytes. The main purpose of melanocytes is the production of melanin, the protein responsible for the pigmentation of the skin, eyes, and hair. Melanin protects the cells in the skin and in deeper layers from the hazardous effects of UV radiation. It is produced and stored in melanosomes, which are located close to the melanocyte membrane, but it can also be transferred to neighboring keratinocytes.

Advantages:

• Isolated in BPE-free, serum-free and PMA-free Melanocyte Growth Medium M3
• No adverse effects of PMA (Phorbol Myristate Acetate) on cell metabolism
• Each produced lot is tested for growth performance up to 15 population doublings, adherence rate, viability, and characteristic dendritic cell morphology
• Guaranteed cell expansion performance when cultured in Melanocyte Growth Medium M3

Our Normal Human Epidermal Melanocytes M3 are isolated using the serum-free, BPE-free and PMA-free Melanocyte Growth Medium M3. Since PMA (Phorbol Myristate Acetate) is a tumor promoting mitogen, it can interfere with experimental approaches. Therefore, we recommend using cells isolated in Melanocyte Growth Medium M3 and also using this medium for cultivation.

• Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Dermal Fibroblasts (NHDF) from the same donor are available on request.
• Our NHEM M3 are also available from HLA-typed donors.

*The catalog numbers in this table are for media in ready-to-use packaging.