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Human M2 Macrophages (M-CSF) monocyte-derived

Monocyte-derived, polarized, non-activated, primary human macrophages. Polarization in M2-Macrophage Generation Medium XF (incl. M-CSF).

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IV-1555

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Our crypreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.

Cryopreserved human macrophages are produced from monocytes in our well-proven M2 Macrophage Generation Media XF and are available as non-activated, fully qualified M2- (M-CSF-) polarized cells. After 9-10 days of differentiation, the M2 macrophages are cryopreserved using our proprietary, protein-free, and animal-component-free freezing medium, Cryo-SFM or Cryo-SFM Plus. Each cryo vial contains more than 1.5 million or 5 million viable cells after thawing, respectively.

Our M2 Macrophages are now also available from HLA-typed donors.

Figure 1.Culture of our cryopreserved human macrophages. The macrophages can be directly used for experiments after thawing and plating. Optional: activation of polarized M1-/M2 macrophages 24h before assay.

Figure 2.Flow cytometric analysis of our cryopreserved Human M1 and M2 Macrophages. M1 macrophages exhibit a CD68+ (99,74%) and CD80+ (83,33%) marker expression profile, typical for M1 macrophages (upper row). M2 macrophages exhibit a CD68+ (99,72%) and CD163+ (94,91%) marker expression profile, typical for M2 macrophages (lower row).

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