New application note: Wound healing assay kinetic analysis
CytoSMART and PromoCell demonstrate how a wound healing assay can be easily performed with primary human keratinocytes using software-based data analysis to achieve more accurate results….
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A young scientist seeks a method to knock out fibrosis
Lab work is exciting, says Bastian Fischer. Even experiments that just yield their expected results inspire the young German scientist. Fischer is currently working on his PhD, and is investigating how fibrosis develops, so that effective therapies for this medical condition can be found.
A scar is often all that remains of a deep cut – if anything remains at all….
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Cancer organoid co-culture protocol
This application note describes a method for easy 3D organoid culture of Primary Human Dermal Fibroblasts (NHDF) and MCF-7 cells in our Cancer Cell Line Medium XF….
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3D skin model: The promise for dermatology
The development of 3D skin models has revolutionized in vitro studies in dermatology, offering a more representative platform for understanding skin structure and function in health and disease. In this article, we explain the biology of the human skin and explore the transformative potential of 3D skin models in reshaping our understanding of skin function….
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Mimicking the body’s largest organ in vitro with 3D skin models
Researchers are increasingly using 3D cell cultures to study the skin’s features. Three-dimensional skin models offer the potential to study various diseases and test compounds in vitro. Dr. Florian Groeber-Becker from the Fraunhofer Institute for Silicate Research ISC in Wurzburg has developed a highly sophisticated skin model.
It is the first thing people see when they look at us….
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Keratinocytes
Definition and Location Keratinocytes represent the major cell type of the epidermis, the outermost of the layers of the skin, making up about 90 percent of the cells there. They originate in the deepest layer of the epidermis, the stratum basale and move up to the final barrier layer of the skin,…
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Using 3D Skin Models to Test Cosmetics
Skin is the primary focus of cosmetic research. Since the European Union banned animal testing of cosmetics completely in 2013, three-dimensional skin models have been used to evaluate epidermal and dermal responses to cosmetic formulations. Such models provide a reliable tool to assess the functionality and safety of new cosmetic products….
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Studying oxidative stress and skin aging using in vitro human cell culture models
Oxidative stress results from an imbalance of pro- and anti-oxidants and leads to faster skin aging, inflammation and disease. Cell assays and primary cells provide the tools researchers need to study the oxidative process and its association with skin aging….
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Introducing a novel high throughput 3D wound healing model
Non-healing wounds continue to cause significant patient burden. This blog details a novel 3D wound healing model that promises to facilitate the development of new treatments to promote wound healing.
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Upgrade of Our Keratinocyte Cell Culture Portfolio
We have expanded our Keratinocyte Cell Culture Portfolio with the addition of an improved animal-extract free Keratinocyte Growth Medium 3…
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3D cancer model
De-skilling 3D tumor models Tissue-engineered 3D cancer models have grown in popularity with recent advances in cancer research. 3D models are more biomimetic than 2D cell monolayers cultured on tissue culture plastic. Examples are spheroids, tumorspheres, organoids, PDX and lately, tumoroids, which differ by origin (cell line or patient-derived), size, the complexity of cellular architecture and tumor environment….
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Antibiotics in Cell Culture: Friend or Enemy?
Antibiotics are routinely used in cell cultures to prevent bacterial infections. But there are side effects: Studies show that they impair cell growth and differentiation. With good laboratory practice, the use of antibiotics is unnecessary.
A cell culture is a world of its own. The culture dish contains nutrients within the media that cells need to thrive and prosper….
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