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Some of our customers have successfully used PromoCell Endothelial Cell Growth Medium MV2 (C-22022) when co-culturing Human Coronary Artery Endothelial Cells (HCAEC; C-12221) and Normal Human Dermal Fibroblasts (NHDF; C-12300).
Both cytokines are produced in E.coli.
The pH of our Endothelial Cell Basal Media MV/MV2 is 7.4 ± 0.1.
The source of our heparin is ex porcine mucosa.
PromoCell's Endothelial Cell Growth Medium (C-22010) is a complete medium that can be used for the culture of HUVEC after addition of the SupplementMix.
Addition of extra FCS is not necessary. The SupplementMix contains FCS (2% v/v final concentration); recombinant growth factors; hormones; and a bovine brain extract that together have mitogenic effects on endothelial cells.
PromoCell's HDLEC are isolated from skin (dermis). When isolated from juvenile donors (C-12216); the exact localization is foreskin. When derived from adult donors (C-12217); the localization depends on the type of surgery; e.g. breast or temple. You can find the information on the exact localization in the Certificate of Analysis.
Our HDLEC are tested to be positive for CD31; podoplanin; and Prox-1 and are delivered in P2. The recommended culture medium is Endothelial Cell Growth Medium MV2 (C-22022).
Epidermal Growth Factor (EGF) and hydrocortisone have been reported to have a synergistic effect on the growth of microvascular endothelial cells. Accordingly if you remove the hydrocortisone the proliferation of HDMEC is clearly affected.