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PromoCell's Endothelial Cell Growth Medium (C-22010) is a complete medium that can be used for the culture of HUVEC after addition of the SupplementMix.
Addition of extra FCS is not necessary. The SupplementMix contains FCS (2% v/v final concentration); recombinant growth factors; hormones; and a bovine brain extract that together have mitogenic effects on endothelial cells.
Epidermal Growth Factor (EGF) and hydrocortisone have been reported to have a synergistic effect on the growth of microvascular endothelial cells. Accordingly if you remove the hydrocortisone the proliferation of HDMEC is clearly affected.
The standard medium for isolation and propagation of our HUVEC HUAEC HPAEC and HSaVEC is Endothelial Cell Growth Medium (C-22010). It contains ECGS an extract from bovine hypothalamus which has mitogenic effects on endothelial cell proliferation. Scientists who prefer a more defined Growth Medium can use Endothelial Cell Growth Medium 2 (C-22011). In this medium ECGS is replaced by VEGF IGF and additional bFGF and EGF to stimulate endothelial cell growth.