Melanocyte Growth Medium M3

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Our unique Melanocyte Growth Medium M3 is designed for the isolation and cultivation of human melanocytes from human juvenile and human adult skin in a BPE- and serum-free environment. The medium does not contain PMA (Phorbol Myristate Acetate), which could interfere with some experimental settings. Cells cultured in this medium can be plated directly on tissue culture plastic and no matrix coating is needed. The optimized universal formulation for juvenile and adult melanocytes show robust growth kinetics without the addition of further growth factors to achieve at least 15 population doublings for all PromoCell tissue donors.  Isolated human juvenile and adult melanocytes show their characteristic dendritic morphology when cultured in this advanced cell culture medium.

Innovation highlight:

We are the first to offer a BPE- and serum-free melanocyte growth medium, setting a new benchmark for standardization and reproducibility in melanocyte culture.

Advantages:

• No adverse effects of PMA (Phorbol Myristate Acetate) on experimental settings
• Each produced media lot is tested for growth promoting activity
• Characteristic dendritic cell morphology
• Compatible with all available PromoCell Melanocytes (NHEM) isolated from different skin areas (e.g. epidermis of breast, eyelid, labia or forskin tissue)
• Using Melanocyte Growth Medium M3 with or without phenol red guarantees at least 15 populations doublings of juvenile and adult NHEM concerning to our protocol
• Our NHEM M3 show stable TRP1 (Tyrosinase related protein 1) levels, which is part of the melanin synthesis pathway,  through at least 15 population doublings  indicating functional active pigment cells
• Regulatory-friendly with phenol red-free version of our ready-to-use Melanocyte Growth Medium M3

If you require this product for further manufacturing in a regulated environment, please request our Custom GMP Services.