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Melanocyte Growth Medium M3
Serum-free and BPE-free cell culture medium for the cultivation of juvenile and adult human melanocytes. Formulation is PMA-free.
Unit
1 Mix for 500 ml
Cat.No.
IV-8669
From
220.00$
Our Melanocyte Growth Medium M3 is designed for the isolation and cultivation of human melanocytes from human juvenile and human adult skin in a BPE- and serum-free environment. The medium does not contain PMA (Phorbol Myristate Acetate), which could interfere with some experimental settings. Cells cultured in this medium can be plated directly on tissue culture plastic and no matrix coating is needed. Isolated human juvenile and adult melanocytes show their characteristic dendritic morphology when cultured in this advanced cell culture medium.
Advantages:
- Highest degree of standardization achieved by BPE-free and serum-free formulation
- No adverse effects of PMA (Phorbol Myristate Acetate) on experimental settings
- Each produced media lot is tested for growth promoting activity and characteristic dendritic cell morphology
- Compatible with all available PromoCell Melanocytes (NHEM)
Figure 1. Amount of TRP1 positive cells at P6 (~15 population doublings).
Figure 2.Representative FACS plot of TRP1 analysis of NHEM-f M3 cultured in Melanocyte Growth Medium M3 (prf). Upper panel: anti-TRP1 stained cells. Lower panel: unstained cells as gating strategy for TRP1 negative cells.
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